Shameik Moore

Miles Morales (voice)

Hailee Steinfeld

Gwen Stacy (voice)

Brian Tyree Henry

Jefferson Morales (voice)

Luna Lauren Vélez

Rio Morales (voice)

Oscar Isaac

Miguel O'Hara (voice)

Jake Johnson

Peter B. Parker (voice)

Jason Schwartzman

Spot (voice)

Issa Rae

Jessica Drew (voice)

Daniel Kaluuya

Hobie Brown (voice)

Nicolas Cage

Spider-Man Noir (voice)

John Mulaney

Spider-Ham (voice)

Karan Soni

Pavitr Prabhakar (voice)

Kimiko Glenn

Peni Parker (voice)

Amandla Stenberg

Margo Kess (voice)

Mahershala Ali

Uncle Aaron (voice)

Jharrel Jerome

Miles G. Morales (voice)

Greta Lee

LYLA (voice)

Andy Samberg

Ben Reilly (voice)

Shea Whigham

George Stacy (voice)

Marvin Jones III

Tombstone (voice)

Liev Schreiber

Wilson Fisk (voice)